Detailed Schedule Weinstein Conference 2010


Great Hall of the Royal Tropical Institute, Amsterdam, the Netherlands


Thursday 20 May 2010

8.00 - 10.00


+ tea/coffee

+ putting up posters for session A

10.10 - 10.20 

Welcome and Opening

Maurice van den Hoff (Local organizing committee chair)


Chairs: Phil Barnett & Tony Firulli

10.20 -10.40

Conditional Disruption of Phosphoregulation of Hand1 Results in Outflow Tract Defects

Beth Firulli*, Joshua W. Vincetz, Ralston M. Barnes, Anthony B. Firulli


10.40 - 11.00

Tbx2 and Tbx3 induce cushion formation and are required for AVC patterning

Reena Singh*, Willem M.H. Hoogaars, Thomas Grieskamp, Sameer Rana, Vincent M. Christoffels and Andreas Kispert


11.00 - 11.20

The Cardiac Transcription Network Driven by the Interplay of Transcription Factors, Histone Modifications and microRNAs

Jenny Schlesinger*, Markus Schueler, Marcel Grunert, Jenny J. Fischer, Qin Zhang, Tammo Krueger, Martin Lange, Martje Tönjes, Ilona Dunkel, Silke R. Sperling


11.20 - 11.40

A MEF2C-Myocardin complex directly regulates the cardiac metabolic master regulatory gene Ampk α2 through a conserved myocardial-specific enhancer

Courtney M. Anderson*, Analeah B. Heidt, Shan-Mei Xu, Brian L. Black


11.40 - 12.00

Inhibition of cardiac myocyte apoptosis by Zac1, an essential cardiac transcription factor

Shinsuke Yuasa*, Takahiko Nishiyama, Owen W.J. Prall, Richard P. Harvey, Keiichi Fukuda


12.00 - 14.00

Lunch + Poster session A


Chairs: Vincent Christoffels & Brian Black

14.00 - 14.20

Hand2 loss-of-function in Hand1-expressing cells Reveals Distinct Roles In Heart And Coronary Vascular Development

Ralston M. Barnes*, Beth A. Firulli, Josh W. Vincentz, Peter Cserjesi, Simon J. Conway, Anthony B. Firulli


14.20 - 14.40

Direct Reprogramming of Cardiac Fibroblasts into Functional Cardiomyocytes by Defined Factors

Masaki Ieda*, Jidong Fu, Vasanth Vedantham, Benoit G. Bruneau, and Deepak Srivastava


14.40 - 15.00

Sox4 cooperates with Tbx3 in the regulation of T-box-gene targets

C.J.J. Boogerd*, L.Y.E. Wong, M. van den Boogaard, M.L. Bakker, P.A.C. ’t Hoen, A.F. Moorman, V.M. Christoffels and P. Barnett.


15.00 - 15.20

Prdm1, encoding the Blimp1 transcriptional repressor, genetically interacts with Tbx1 during distal outflow tract morphogenesis.

Stéphane D. Vincent*, Sachiko Miyagawa-Tomita and Margaret Buckingham


15.20 - 15.40

A Direct microRNA Target Screen Reveals Alternate Regions of Complementarity that Mediate Repression in cardiac homeostasis

Eva Samal*, Vasanth Vedantham, Ru-Fang Yeh, Nathan Salomonis, YuanYuan Xiao, Morgan Von-Drehle, Linda van Laake, Yong Zhao, David J. Erle Deepak Srivastava


15.40 - 16.20



Chairs: Christine Mummery & Scott Baldwin

16.20 - 16.40

Endothelial progenitors contribute to the heart endocardium and play key roles in cardiac development

Michal Milgrom-Hoffman*, Napoleone Ferrara, Elazar Zelzer and Eldad Tzahor


16.40 - 17.00

Identification of micro RNAs involved in cardiac specification in mESCs

Alexandre Colas*, Wesley McKeithan, Mark Mercola


17.00 -17.20

Regulation of cardiac progenitor cells during heart development

Francesca Rochais* and Robert G. Kelly


17.20 - 17.40

Hematopoietic cell contribution to embryonic mouse valve development and adult valve homeostasis

Zoltan Hajdu*, Roger R Markwald, Christopher C Drake, Imre Olah, Richard P Visconti


17.40 - 18.30


Chair: Maurice van den Hoff

Prof Denis Duboule:             Genetic Control of Vertebrate                                                        Morphogenesis and Evolution

University of Geneva, Geneva & School of Life Sciences, Lausanne, Switzerland.

18.30 - 19.30

ESC-WG DAP Meeting

18.30 - 20.00


20.00 - 23.00

Buffet dinner

+ taking down of Posters of session A


Friday 21 May 2010

8.00 - 9.00


+ putting up posters for session B

8.00 - 9.00

Weinstein Business meeting

Platform session EPICARDIUM

Chairs: Thomas Brand & Takashi Mikawa

9.00 - 9.20

Epicardial fate and Notch signaling

Thomas Grieskamp, Carsten Rudat, Julia Norden, Andreas Kispert*


9.20 - 9.40

Loss of the Type III Transforming Growth Factor-β Receptor decreases Hyaluronic Acid responsiveness in Epicardial Cells

Nora S. Sánchez*, Evisabel Craig, Joseph D. Love, Todd Camenisch, and Joey V. Barnett


9.40 - 10.00

NFATc1 in epicardium-derived cells is required for fibrous skeleton and intramyocardial coronary vessel development

Michelle D. Combs*, Caitlin M. Braitsch, and Katherine E. Yutzey


10.00 -10.20

PDGF Signaling is Required for Epicardial Functions and New Blood Vessel Formation in Regenerating Zebrafish Hearts

Qiong Wu, Yolanda Zhang, Jieun Kim, Katie M. Wiens, Hiro Shimada, Robert I. Handin, Michael Y. Chao, Tai-Lan Tuan, Vaughn A. Starnes and Ching-Ling Lien*


10.20 - 10.40

PDGFR signaling in the epicardium is required for epicardial EMT and coronary VSMC and cardiac fibroblast development

Christopher L. Smith*, Seung Tae Baek,and Michelle D. Tallquist


10.40 - 11.20



Chairs: Paul Riley & Richard Harvey

11.20 - 11.40

Inhibition of Bmp signaling by Smad6 is required for terminal differentiation of ventricular cardiomyocytes.

Emma de Pater and Jeroen Bakkers*


11.40 - 12.00

The BMP Pathway Acts to Regulate Tbx20 in the Developing Heart

Erin Kaltenbrun*, Elizabeth Mandel, Thomas Callis, Da-Zhi Wang and Frank Conlon


12.00- 12.20

Bmp-signaling regulates myocardial differentiation from cardiac progenitors through a micro RNA-mediated mechanism

Jun Wang*, Margarita Bonilla-Claudio, Jue Zhang, Yan Bai, Zheng Huang, Brian L. Black, Fen Wang, and James F. Martin


12.20 -12.40

Cardiac Laterality is Controlled by Multiple Rho Kinase Signaling Pathways in Ciliated Cells

Guangliang Wang, Fiona C. Foley, Duck Soo Jang, Jeffrey D. Amack*


12.40 - 13.00

Mitochondrial structure and function during cardiac myocyte differentiation

George A. Porter, Jr.*, Jennifer Hom, Rodrigo Quintanilla, David Hoffman, Bentley Karen, Shey-Shing Sheu


13.00 - 14.30

Lunch + Poster session B



Chairs: Diego Franco & Alex Postma

14.30 - 14.50

Dysregulation of the PDGFRA gene causes inflow tract anomalies including TAPVR: Integrating evidence from human genetics and model organisms

Steven B. Bleyl*, Yukio Saijoh, Noortje A.M. Bax, Adriana C. Gittenberger-de Groot, Lambertus J. Wisse, Shigehito Yamada, Kohei Shiota and Gary C. Schoenwolf.


14.50 - 15.10

Effects of conditional deletion of hypoxia-inducible factor (hif) and hypoxic stress on mouse heart morphogenesis

Hongbin Liu and Steven A. Fisher*


15.10 - 15.30

p53-related p63 transcription factor is essential for cardiac morphogenesis

Alain Medawar, Matthieu Rouleau, Laurent Hamon, Huiqing Zhou, Hans van Bokhoven, Caterina Missero, Eleonora Candi, Cedric Blanpain, Gerry Melino, Michel Puceat and Daniel Aberdam*


15.30 - 15.50

Copy Number variations (CNV) in left sided congenital heart disease

Marc-Phillip Hitz*, Louis-Philippe Lemieux-Perreault, Christian R. Marshall, Bert Overduin, Maryse Thibeault, Alexandre F. R. Stewart, Stephen W. Scherer, Andrea Richter, Marie-Pierre Dubé, Gregor Andelfinger


15.50 - 16.10

The role of FKbp12-notch1 mediated regulation in ventricular trabeculation and compaction

Hanying Chen*, Wenjun Zhang, Xiao-Xin Sun, Zhuang Chen, Kryn Stankunas, Yongzhen He, Weidong Yong, Wuqiang Zhu, Simon Conway, Ching-Pin Chang, Loren J. Field, Nadia Carlesso, Weinian Shou


16.10 - 16.30

MCTP2 is a novel gene causing aortic coarctation

S.M. Ware*, X. Wang, L. Potocki, G. Zapata, M. Bray, A.C. Chinault, B.A. Boggs, E.K. Brundage, J.A. Towbin, A. Patel, S.D. Fernbach, S.L. Hamilton, K.L. McBride, J.W. Belmont, S.R. Lalani.


16.30 - 17.00

Break + taking down of  Posters of session B

Netherlands Heart Foundation Technical session 3D AND IMAGING TECHNIQUES

Chairs: Jan Ruijter & Tim Mohun

17.00 - 17.20

3D Modelling of heart development: looking to the future

Tim Mohun


17.20 - 17.40

Imaging in the Embryonic Heart: morphodynamic studies with Optical Coherence Tomography (OCT)

Mesud Yelbuz


17.40 - 18.00

How to quantify in 3D: 3D imaging of gene expression patterns and morphogenetic parameters in the embryonic heart.

Jan M. Ruijter*, Alexandre T. Soufan, Jaco Hagoort, and Antoon F.M. Moorman


18.00 - 18.20

How to place a section: automated fitting of histological sections into a high resolution 3D reference model of a developing mouse heart

Bouke A de Boer*, Frans PJM Voorbraak, Maurice JB van den Hoff, Antoon FM Moorman, Jan M Ruijter


18.20 - 18.40

How to make a 3D pdf: from 3D-reconstruction to interactive 3D-pdf; a protocol for biologists.

Jaco Hagoort*, Alexandre Soufan, Bouke de Boer, Antoon Moorman


18.45 -

Free evening + taking down of  Posters of session B

Saturday 22 May 2010

8.00 - 9.00


+ putting up posters for session C

8.00 - 9.00

NIH Research Partnerships at Home and Around the World

Dr Charlene Schramm

Platform session VALVE DEVELOPMENT

Chairs: Katherine Yutzey & Andy Wessels

9.00 - 9.20

MiR-23 is essential to restrict endothelial-to-mesenchymal transition during cardiac valve formation.

Anne Karine Lagendijk*, Jeroen Bakkers


9.20 - 9.40

Cardiac valve malformations: new insights from Pdlim7, an unexpected suspect in heart development

Jennifer Krcmery*, Rudyard Sadleir, Rajesh Gupta, Chrissy Kamide, Sol Misener, Doug Losordo, and Hans-Georg Simon


9.40 - 10.00

Krox20 is required during valve remodeling and maturation

Sarah Arab, Frank Kober, Piotr Topilko, Monique Bernard, Patrick Charnay, Patrick Cozzone and Stéphane Zaffran*


10.00 -10.20

The Metalloprotease ADAMTS5 Plays A Critical Role In ECM Stratification And Cusp Sculpting During Semilunar Valve Maturation

Christine B. Kern*, Daniel R. McCulloch, Jessica D. McGarity, Alexandra Bahan, Loren E. Danese, Courtney M. Nelson, Andy Wessels, and Suneel S. Apte


10.20 - 10.40

Induction of a developmental gene program in pediatric and adult aortic valve disease

Elaine Wirrig*, Jonathan Cheek, Christina Alfieri, Walter Merrill, Robert Hinton, Katherine Yutzey


10.40 - 11.00

Reduced Sox9 function promotes heart valve calcification in vivo

Jacqueline D Peacock*, Agata K Levay, and Joy Lincoln


11.00 - 11.45


Platform session HEART FIELDS

Chairs: Margaret Buckingham & Jim Martin

11.45 - 12.05

Tbx5 is required in the Second Heart Field for Atrioventricular Septation

Linglin Xie, Joshua M. Friedland-Little, Andrew D. Hoffmann, and Ivan P. Moskowitz*


12.05 - 12.25

Cooperative roles of Tbx1 and Tbx3 during early outflow tract development

Karim Mesbah*, M. Sameer Rana, Laure Lo Ré, Virginia Papaioannou, Vincent M. Christoffels, Robert G. Kelly


12.25 - 13.05


Chair Yigal Pinto

Prof. Frank Grosveld:   Interactions in the genome

Dept of Cell Biology, Erasmus Medical Center, Rotterdam, the Netherlands

13.05 - 14.30

Lunch + Poster session C



Chairs: Margaret Kirby  & Ivan Moskowitz

14.30 - 14.50

Trigenic neural crest-restricted Smad7 over-expression results in congenital craniofacial and cardiovascular defects

Sunyong Tang, Paige Snider and Simon J. Conway *


14.50 - 15.10

Cardiac Neural Crest Nibble Their Way to the Heart

Mary R. Hutson*, Ann Marie Scholl, Elizabeth B. Kuhn, Asako Sato, Harriett A. Stadt, Jennifer R. Decker, Kelly Pegram, and Margaret L. Kirby

*Department of Pediatrics, Neonatal-Perinatal Research Institute, Duke University Medical Center, Durham, NC, USA

15.10 - 15.30

Origins of Cardiac Pacemaking Cells in Avians

Michael Bressan*, Alicia Navetta, Takashi Mikawa


15.30 - 15.50

The role of Tbx3 and Nkx2-5 in the adult atrioventricular conduction system

Mathilda T.M. Mommersteeg*, Bas J. Boukens, Saskia van der Velden, Corrie de Gier-de Vries, Richard P. Harvey, Antoon F.M. Moorman, Vincent M. Christoffels


15.50 - 16.10

Delayed atrioventricular conduction in adrenergic-deficient embryonic mouse hearts: Uncovering a role for retinoic acid

Kingsley Osuala, David G. Taylor, Celines Martinez, Ha-Long Nguyen, Tu-Suong Nguyen, Anupama Natarajan, Peter Molnar, James Hickman, and Steven N. Ebert*


16.10 - 16.40

Break + taking down of  Posters of session C

Platform session CARDIOMECHANICS

Chair: Roger Markwald & Ray Runyan

16.40 - 17.00

Shear stress modulates transcriptional regulation and cushion endoMT in the developing heart

Beerend P. Hierck*, Anastasia D. Egorova, Simone van de Pas, Marie José Goumans, Peter ten Dijke, Robert E. Poelmann


17.00 - 17.20

Integrative computational modeling and imaging approach to study the effects of abnormal hemodynamic conditions on cardiac development.

Sandra Rugonyi*, Ruikang Wang and Kent Thornburg


17.20 - 17.40

Knockdown of embryonic myosin heavy chain causes defective electrical activities and calcium signalling within the developing heart

Catrin Rutland, Luis Polo-Parada, Aziza Alibhai, Aaran Thorpe and Siobhan Loughna*


17.40 -17.50

Diego Franco: presentation of the Pexieder Award

17.50 - 18.00

Kathrine Yutzey Introducing the Weinstein Conference 2011 in Cincinnati

18.00 - 18.10

Closure of the Scientific Part of the Meeting

18.10 -18.20

Taking down of  Posters of session C

18.20 - 18.30


18.30 - 20.00

"Floating wine and cheese party"

Boat trip through the canals of Amsterdam to the Hotel Barbizon Palace

20.00 - 01.00

Farewell dinner party in the Olaf chapel (Hotel Barbizon Palace)